Equilibrium dialysis studies on two lima bean lectins
نویسندگان
چکیده
منابع مشابه
Lectins in castor bean seedlings.
The amounts of the two lectins (ricin and Ricinus communis agglutinin) in tissues of castor bean seedlings were followed during germination and early growth. For measurement, lectins in extracts were separately eluted from Sepharose columns; an antibody to the agglutinin was also used to detect the lectins by immunodiffusion. The endosperm of the dry seed contains 3.5 mg total lectin (5.6% of t...
متن کاملEquilibrium dialysis and cell binding studies on Bandeiraea simplicifolia lectin.
Equilibrium dialysis studies on the binding of the blood group B-specific Bandeiraea simplicifolia lectin to methyl alpha-D-galactopyranoside demonstrated the existence of one carbohydrate binding site per subunit for the tetrameric protein, with an intrinsic association constant of 8.6 x 10(4) (M) (-1) at 2 degrees and 3.3 x 10(4) (M)(-1) at 20 degrees. These values correspond to a free energy...
متن کاملGlycosyl moiety of the lima bean lectin.
Pronase digestion of the lima bean lectin (Phaseolus lunatus (alpha-D-GalNAc greater than alpha-D-Gal)) afforded a glycopeptide of molecular weight 1380. Amino acid and carbohydrate analysis showed the glycopeptide to contain fucose, mannose, glucosamine, and aspartic acid in the molar ratios, 0.8:4.4:1.9:1.0. Methylation analysis, Smith degradation, and sequential degradation by specific glyco...
متن کاملPhotoaffinity labeling of the adenine binding site of the lectins from lima bean, Phaseolus lunatus, and the kidney bean, Phaseolus vulgaris.
8-Azidoadenine was employed as a photoaffinity probe of the adenine binding site of the seed lectin from lima beans and from Phaseolus vulgaris erythroagglutinin. This compound was shown to (a) bind competitively to the adenine binding site of these lectins and (b) exhibit enhanced binding in the presence of 1,8-anilinonaphthalenesulfonic acid in the same manner as adenine. The presence or abse...
متن کاملProtein Binding Studies of Gossypin by Equilibrium Dialysis
Gossypin is a glucosyl flavone obtained from the flowers of Hibiscus pitifolius (Malvaceace). It has potent analgesic and antiinflammatory activity. It has shown cytotoxic activity when tested against human lung adrenocarcinoma cell lines (A549). It has been shown to suppress angiogenesis, inflammation, and carcinogenesis. The mechanisms of these activities, however, are unknown. Because of the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 1974
ISSN: 0003-9861
DOI: 10.1016/0003-9861(74)90184-2